abstract：AIM:To study the effect of chronic insulin treatment on insulin post-receptor signaling transduction and whether the effects of metformin are transmitted throughout the cascade of insulin signaling intermediates in a human hepatoma cell line (Hep G2). METHODS:Hep G2 cells were incubated in serum free media containing ...

journal_title：Acta pharmacologica Sinica

authors： Yuan L,Ziegler R,Hamann A

更新日期：2003-01-01 00:00:00

abstract：AIM:To test the ability of huperzine B (HupB) to alleviate injury from oxygen-glucose deprivation (OGD) in the rat pheochromocytoma line PC12 cells. METHODS:After OGD for 3 h and reoxygenation for 24 h, neuronal morphology was observed by phase-contrast microscopy; cell survival was quantified by the reduction of MTT;...

journal_title：Acta pharmacologica Sinica

authors： Wang ZF,Zhou J,Tang XC

更新日期：2002-12-01 00:00:00

abstract：AIM:To study the effects of tetrandrine (Tet) on extracellular Ca2+ influx and intracellular Ca2+ release in cultured calf basilar artery smooth muscle cells. METHODS:Free intracellular calcium was examined by a system of measurement of AR-CM-MIC, using Fura 2-AM as a fluorescent indicator. RESULTS:In the presence of...

journal_title：Acta pharmacologica Sinica

authors： Wang B,Xiao JG

更新日期：2002-12-01 00:00:00

abstract：AIM:To evaluate the protective effects of apocynin on "two-hit" injury in rats. METHODS:"Two-hit" injury model of rat was induced by hemorrhagic shock (40 mmHg for 45 min) followed by iv administration of lipopolysaccharide (LPS, 150 microg/kg). Rats were randomized into seven groups: Sham, LPS, hemorrhage, hemorrhage...

journal_title：Acta pharmacologica Sinica

authors： Zhou R,Hu DY,Liu LM,Zhou XW

更新日期：2002-11-01 00:00:00

abstract：AIM:To examine the role of cholinergic neurons in the nucleus raphe magnus (NRM) in noxious heat stimulation and in the effects of morphine-induced antinociception by rats. METHODS:After the cholinergic neuron selective toxin, AF64A, was microinjected into the NRM, we examined changes in the antinociceptive threshold ...

journal_title：Acta pharmacologica Sinica

authors： Abe K,Ishida K,Kato M,Shigenaga T,Taguchi K,Miyatake T

更新日期：2002-11-01 00:00:00

abstract：AIM:To study the effect of new antihepatitis drug, bicyclol, on the metabolism and hepatotoxicity of aflatoxin B1 (AFB1) in rats. METHODS:Rats were given bicyclol 300 mg/kg/d ig for 3 d and then injected ip with AFB1 1.5 mg/kg. Liver damages were examined 16 h after ip AFB1. The in vitro metabolism of AFB1 by bicyclol...

journal_title：Acta pharmacologica Sinica

authors： Lu H,Li Y

更新日期：2002-10-01 00:00:00

abstract：AIM:To determine whether ONO-1078 (pranlukast), a potent leukotriene receptor antagonist, has neuroprotective effect on focal cerebral ischemia in the rat. METHODS:Focal cerebral ischemia was induced by 30 min of middle cerebral artery (MCA) occlusion and followed by 24 h reperfusion. ONO-1078 (0.003-1.0 mg/kg) or veh...

journal_title：Acta pharmacologica Sinica

authors： Zhang WP,Wei EQ,Mei RH,Zhu CY,Zhao MH

更新日期：2002-10-01 00:00:00

abstract：AIM:To study the alterations of myocardial taurine transport function, taurine transporter (TAUT), and cysteine sulfinate decarboxylase (CSD) mRNA as well as effect of exogenous taurine in rats with isoproterenol (ISO)-induced cardiomegaly. METHODS:[3H]-Taurine uptake and release on myocardium were determined. Binding...

journal_title：Acta pharmacologica Sinica

authors： Shi YR,Bu DF,Qi YF,Gao L,Jiang HF,Pang YZ,Tang CS,Du JB

更新日期：2002-10-01 00:00:00

abstract：AIM:To study the protective effect of the polypeptide isolated from Chlamys farreri (PCF) on hairless mice skin damaged by ultraviolet A. METHODS:Enzymes and malondialdehyde (MDA) were determined by biochemical methods; the expressions of Bcl-2 protein and NOS protein were examined by immunohistochemical technique. Th...

journal_title：Acta pharmacologica Sinica

authors： Wang CB,Yao RY,Liu ZT,Zhong WZ,Liu XP,Wang YJ

更新日期：2002-09-01 00:00:00

abstract：AIM:To study the effect of Huangqi Jianzhong Tang in reducing fatigue improvement in athletes. METHODS:Twelve athletes were randomly separated into a control group, whose members were administered with placebos, and the experimental group, whose members were administered with Huangqi Jianzhong Tang. During the eight w...

journal_title：Acta pharmacologica Sinica

authors： Chen KT,Su CH,Hsin LH,Su YC,Su YP,Lin JG

更新日期：2002-08-01 00:00:00

abstract：AIM:To investigate the effects of different muscarinic receptor (M) subtypes in the spinal cord on the scores of naloxone-precipitated morphine-withdrawal symptoms and the changes of nNOS expression in locus coeruleus (LC). METHODS:nNOS immunohistochemistry, intrathecal injection (it), and antisense oligonucleotides (...

journal_title：Acta pharmacologica Sinica

authors： Chou WB,Zeng YM,Duan SM,Zhou WH,Gu J,Yang GD

更新日期：2002-08-01 00:00:00

abstract：AIM:To determine whether basophils expressed human leucocyte antigen (HLA) class II molecules. METHODS:Basophils in umbilical cord blood were separated and purified with methods of density gradient centrifugation and immunomagnetic microbeads. The isolated basophils were cultured in RPMI-1640 plus 10 % fetal calf seru...

journal_title：Acta pharmacologica Sinica

authors： Xie ML,Wu YG

更新日期：2002-07-01 00:00:00

abstract：AIM:To search for novel effective P-glycoprotein (P-gp) reversal agents in the blood-brain barrier (BBB). METHODS:Using rhodamine123 (Rh123) to examine the functional activity of P-gp in cultured bovine brain capillary endothelial cells (BCEC) and screen various principles on P-gp modulation in BBB. RESULTS:All of te...

journal_title：Acta pharmacologica Sinica

authors： He L,Liu GQ

更新日期：2002-07-01 00:00:00

abstract：AIM:To study the effect and mechanism of pyronaridine (PND) on the reversal of multidrug resistance (MDR) in K562/A02 and MCF7/ADR cell lines with mdr1+. METHODS:MTT assay was used to determine the cells growth inhibition after incubation for 72 h in the presence of doxorubicin (DOX) with or without PND. Intracellular...

journal_title：Acta pharmacologica Sinica

authors： Qi J,Yang CZ,Wang CY,Wang SB,Yang M,Wang JH

更新日期：2002-06-01 00:00:00

abstract：AIM:To differentiate the electrophysiological characteristics of somatic action potentials (AP) from isolated Neo and Juv nodose sensory neurons (NSN) and those from slices of intact Juv and adult rat nodose ganglia. METHODS:For isolated cell recordings nodose ganglia from 3-8 d old Neo and 4 weeks old Juv rats were d...

journal_title：Acta pharmacologica Sinica

authors： Li BY,Schild JH

更新日期：2002-06-01 00:00:00

abstract：AIM:To investigate the metabolic characteristics of cytochrome P-450 C YP2A6 in human liver microsomes. METHODS:Cytochrome P-450 enzyme activities were measured by biochemical assays. Xenobiotics were employed to observe their effects on CYP2A6 in vitro. The kinetics of coumarin 7-hydroxylase was determined, and the c...

journal_title：Acta pharmacologica Sinica

authors： Xia XY,Peng RX,Yu JP,Wang H,Wang J

更新日期：2002-05-01 00:00:00

abstract：AIM:To study the expressions of vascular endothelial growth factor (VEG F), angiopoietin-1 (Ang-1), angiopoietin-2 (Ang-2), Tie-1, and Tie-2 in C57BL/6 mouse brain after permanent focal cerebral ischemia. METHODS:The mRNA levels of VEGF, Ang-1, Ang-2, Tie-1, and Tie-2 were measured by semiquantitative reverse transcri...

journal_title：Acta pharmacologica Sinica

authors： Wang RG,Zhu XZ

更新日期：2002-05-01 00:00:00

abstract：AIM:To study the effect of semi-synthesized quercetin water-soluble derivatives sodium quercetin-7-sulfate (SQMS) and disodium quercetin-7,4 -disulfate (SQDS) on recombinant human phosphatidylinositol 3-kinase (PI3-K) p110 beta catalytic subunit. METHODS:Recombinant human PI3-K p110 beta catalytic subunit was expresse...

journal_title：Acta pharmacologica Sinica

authors： Liu W,Liang NC

更新日期：2002-04-01 00:00:00

abstract：AIM:To examine the effect of tachykinins on the ascending reflex pathway in rat small intestine, we used different selective neurokinin (NK) receptor antagonists (RA): a) NK1-RA: GR-82334 and CP-96.345, b) NK2-RA: MEN-10.376 and L-659.877. The aim was further to investigate the effect of substance P (SP) on the ascendi...

journal_title：Acta pharmacologica Sinica

authors： Hahn A,Storr M,Allescher HD

更新日期：2002-04-01 00:00:00

abstract：AIM:To explore correlation of T helper 1 (Th1) and Th2 cytokine between the cortical layer and pulmonary airway in ovalbumin-induced rat asthma model. METHODS:Aerosol antigen-induced changes of inflammation in bronchoalveolar lavage fluids (BALF) and pulmonary histologic section in sensitized rats were investigated. C...

journal_title：Acta pharmacologica Sinica

authors： Xie QM,Chen JQ,Shen WH,Bian RL

更新日期：2002-03-01 00:00:00

abstract：AIM:To isolate the G4 and G1 molecular forms of acetylcholinesterase in rat cortex for interpreting the therapeutical effect of the AChE inhibitor drugs. METHODS:Size exclusion chromatography and ultracentrifugation were used to isolate and ascertain the G4 and G1 molecular forms of AChE. RESULTS:After size exclusion...

journal_title：Acta pharmacologica Sinica

authors： Zhao Q,Tang XC

更新日期：2002-02-01 00:00:00

abstract：AIM:To establish a 96-well plate based kinase assay using a recombinant vascular endothelial growth factor (VEGF) receptor 1 kinase domain protein. METHODS:A human VEGF receptor 1 kinase domain protein was expressed in E coli, and its activity was monitored by its ability of phosphorylating the polyE4Y substrate coate...

journal_title：Acta pharmacologica Sinica

authors： Zhuang SF,Zhou CH,Qian J,Qian Z,Shibuya M,Ye QZ

更新日期：2002-02-01 00:00:00

abstract：AIM:To investigate whether or not brain histamine was involved in memory deficits induced by lesions of nucleus basalis magnocellularis (NBM) in rats. METHODS:Passive avoidance response was used to measure memory process in rats, and NBM was bilaterally lesioned by injection of ibotenic acid (6 microg/site). RESULTS:...

journal_title：Acta pharmacologica Sinica

authors： Chen Z,Shen YJ

更新日期：2002-01-01 00:00:00

abstract：AIM:To present the 4 to 9 years (median: 6 years) treatment follow up of 10 HIV1-AIDS patients, 9 at AIDS and 1 at A3 stages. METHODS:We have applied from 1992 to 1994, AZT combined with 2 integrase inhibitors, acriflavine and hydroxy-methyl-ellipticine. We could shift, in 1994, to combinations of 3 drugs including tw...

journal_title：Acta pharmacologica Sinica

authors： Mathe G,Morette C,Hallard M,Pontiggia P,Blanquet D,Hage F

更新日期：2002-01-01 00:00:00

abstract：AIM:To study wound healing activity of the human placental extract (HPE) in rats. METHODS:Full thickness wounds were inflicted on depilated dorsum of Charles foster rats with 8 mm Acu-punch biopsy. The HPE was applied both at topical and im routes (2.5 mL/kg). Effects were compared on the basis of physical criteria, b...

journal_title：Acta pharmacologica Sinica

authors： Biswas TK,Auddy B,Bhattacharya NP,Bhattacharya S,Mukherjee B

更新日期：2001-12-01 00:00:00

abstract：AIM:To evaluate the nasal absorption enhancement of insulin by sodium deoxycholate (SDC) in combination with cyclodextrins (CD). METHODS:The concentration of glucose in blood was measured. Scanning electron microscopy technique was used to investigate the effect of enhancers on the nasal mucocilia. The effect of SDC i...

journal_title：Acta pharmacologica Sinica

authors： Zhang Y,Jiang XG,Yao J

更新日期：2001-11-01 00:00:00

abstract：AIM:To examine the effects of microinjection of melatonin and its receptor antagonists into the anterior hypothalamic area (AHA) on blood pressure (BP) and heart rate (HR) in normotensive and stress-induced hypertensive rats. METHODS:Melatonin and its receptor antagonists were microinjected into the AHA, then BP, mean...

journal_title：Acta pharmacologica Sinica

authors： Ding CN,Cao YX,Zhou L,Zhu DN,Shen ZY,Fei MY,Yu P

更新日期：2001-11-01 00:00:00

abstract：AIM:To investigate the stereoselective metabolism of propafenone (PPF) by human liver CYP3A4. METHODS:A chiral and an achiral HPLC were combined to determine the enantiomer of PPF in S9 incubates prepared from transgenic Chinese hamster CHL cells lines expressing CYP3A4. The time-dependent study was performed using in...

journal_title：Acta pharmacologica Sinica

authors： Zhou Q,Yao TW,Yu YN,Zeng S

更新日期：2001-10-01 00:00:00

abstract：AIM:The responses of the intracellular calcium ([Ca2+]i) and the intracellular pH (pHi) to kappa-opioid receptor stimulation were determined in the single right ventricular myocytes isolated from the hearts of chronically hypoxic rats which exhibited right ventricular hypertrophy (RVH). METHODS:With the spectrofluorom...

journal_title：Acta pharmacologica Sinica

authors： Pei JM,Wang YM,Zhu YL,Chen M,Wong TM

更新日期：2001-10-01 00:00:00

abstract：AIM:To study the role of calcitonin gene-related peptide (CGRP) in nitric oxide (NO)-mediated myocardial delayed preconditioning induced by heat stress. METHODS:The isolated rat heart was perfused in a Langendorff model. Hearts for all groups were subjected to 4 h hypothermia (4 degrees C) and 40 min reperfusion (37 d...

journal_title：Acta pharmacologica Sinica

authors： Tan B,He SY,Deng HW,Li YJ

更新日期：2001-09-01 00:00:00

abstract：AIM:To investigate the postantibiotic effects (PAE) of different classes of antimicrobials against five different types of bacteria. METHODS:Minimal inhibitory concentrations (MIC) were determined by twofold macrodilution in broth. The antimicrobial agents were eliminated by washing method after the bacteria were expo...

journal_title：Acta pharmacologica Sinica

authors： Wang MG,Zhang YY,Zhu DM,Zhang J,Shi YG

更新日期：2001-09-01 00:00:00

abstract：AIM:To observe the polymorphism of angiotensin-converting enzyme (ACE) gene in Northern Hans, Dahurs, and Ewenkis of China. METHODS:The polymerase chain reaction was used to type the insertion/deletion polymorphism at intron 16 of ACE gene among 90 Northern Hans, 84 Dahurs, and 64 Ewenkis individuals. The experiment d...

journal_title：Acta pharmacologica Sinica

authors： Zhang YM,Zhang LY,Wang KQ,Ge JB

更新日期：2001-08-01 00:00:00

abstract：AIM:To investigate the effect of melittin (Mel) on papillary muscles of guinea pigs. METHODS:Contraction of papillary muscles were examined by conventional method and action potentials (AP) were recorded by standard glass microelectrode technique. RESULTS:Mel (0.5, 3 micromol/L) significantly increased the contractil...

journal_title：Acta pharmacologica Sinica

authors： Zhang YL,Yang S,Shi NC,Jiang MH

更新日期：2001-08-01 00:00:00

abstract：AIM:To study the regulation of monoamine oxidase-B (MAO-B) activity and imidazoline receptors (I-R) during long term treatment of morphine. METHODS:MAO-B activity was detected by high performance liquid chromatography; I-R was detected by [3H]idazoxan binding test. RESULTS:Idazoxan and morphine inhibited whole brain ...

journal_title：Acta pharmacologica Sinica

authors： Su RB,Li J,Li X,Qin BY

更新日期：2001-07-01 00:00:00

abstract：:Top down central nervous system (CNS) influences on the immune system and bottom up immune system influences on the CNS take part in a complex feedforward and feedback loop which may be responsible for initiating events and perpetuating circumstances in the course of neuropsychiatric as well as immune system diseases....

journal_title：Acta pharmacologica Sinica

authors： Fricchione G,Daly R,Rogers MP,Stefano GB

更新日期：2001-07-01 00:00:00

abstract：AIM:To study in vitro release and in vivo effect of four different types of sustained-release naltrexone microspheres on morphine analgesia. METHODS:Release of naltrexone from four types of biodegradable microspheres was investigated by HPLC. Their antagonist effects on morphine analgesia were observed using mouse hot...

journal_title：Acta pharmacologica Sinica

authors： He GA,Hou HM,Liu XJ

更新日期：2001-06-01 00:00:00

abstract：AIM:To investigate whether low dose theophylline has an anti-inflammatory effect in asthma. METHODS:Nineteen asthmatic subjects were given 200 mg sustained-release theophylline preparation twice daily for 4 weeks. The mean serum concentration of theophylline was 7.9 mg/L. The percentage of eosinophils, EG2+ eosinophil...

journal_title：Acta pharmacologica Sinica

authors： Yang J,Tu HY,Li QQ

更新日期：2001-05-01 00:00:00

abstract：AIM:To study the effects of nimodipine on delayed cerebral injury in mice from subacute carbon monoxide (CO) exposure. METHODS:Mice were exposed to CO (100 mL/kg, ip) once a day, continuously for 7 d. After 7-d CO-exposure, mortality in mice, changes in learning ability and memory using passive avoidance test, the pat...

journal_title：Acta pharmacologica Sinica

authors： Yang JQ,Zhou QX

更新日期：2001-05-01 00:00:00

abstract：AIM:To examine the interactions of allopregnanolone and phenobarbital for the protection against seizures. METHODS:The protective activity of allopregnanolone and/or phenobarbital against seizures was studied in the C57 mice, using the maximal electroshock seizure (MES) test. The modulations by allopregnanolone and/or...

journal_title：Acta pharmacologica Sinica

authors： Lu YQ,Yu R

更新日期：2001-04-01 00:00:00

abstract：:Resibufogenin (RBG) is a single compound isolated from Chansu, a traditional Chinese medicine obtained from the skin venom gland of the toad. Formulations of Chansu have been widely applied in China, Japan, and other Asian countries for a long time and are currently used as alternative medicines. However, there have b...

journal_title：Acta pharmacologica Sinica

authors： Xie JT,Dey L,Wu JA,Lowell TK,Yuan CS

更新日期：2001-04-01 00:00:00